MOLECULAR BIOLOGY

The Molecular Biology Laboratory (LBM) is part of IRNASA’s research support services. It collaborates and participates in the Institute’s research projects in which Molecular Biology is one of the participating disciplines and also offers its services to external users (PRIs, universities, private companies, etc.), providing them with scientific and technical advice.

The LBM integrates the technology and software necessary for the development of its three pillars: biotechnology, molecular biology and biostatistical analysis of laboratory results.

Production of recombinant proteins

or metabolites of interest

Real-time PCR

Biostatistical and bioinformatics support

Liquid chromatography

Scientific and technical advice

THE LBM OFFERS THE FOLLOWING SERVICES:

  • CARRYING OUT MEDIUM-SCALE FERMENTATION PROCESSES FOR THE PRODUCTION OF RECOMBINANT PROTEINS OR METABOLITES OF INTEREST.

  • REAL-TIME PCR (RT-PCR) FOR NUCLEIC ACID QUANTIFICATION (TRANSCRIPT LEVELS, ETC.), MUTATION DETECTION AND PROTEIN STABILITY STUDIES.

  • BIOSTATISTICAL AND BIOINFORMATICS SUPPORT, INCLUDING THE NECESSARY EQUIPMENT FOR THE ACQUISITION OF IMAGES OF GELS AND BLOTS, AND FOR THE ANALYSIS OF RESULTS FROM THESE SUPPORTS.

  • LIQUID CHROMATOGRAPHY (LOW PRESSURE FPLC) DESIGNED FOR FAST AND RELIABLE SEPARATIONS OF PEPTIDES, NUCLEIC ACIDS, OLIGONUCLEOTIDES AND PROTEINS.

  • SCIENTIFIC AND TECHNICAL ADVICE.

THE FOLLOWING EQUIPMENT AND SOFTWARE ARE AVAILABLE FOR THIS PURPOSE:

  • MEDIUM-SCALE FERMENTATION PLANT, CONSISTING OF:

    • Applikon BioBench 30 litre fermenter.

    • Semi-continuous centrifuge CEPA LE (30 litres/hour).
    • Homogeniser Emulsiflex-C3 (3 litres/hour).

  • APPLIED BIOSYSTEMS MODEL 7900HT REAL-TIME PCR KIT.

  • BIONUMERICS SOFTWARE 6.6.4.

  • CHEMIDOC MP SYSTEM BLOT AND GEL IMAGING EQUIPMENT, ASSOCIATED WITH IMAGELAB AND PDQUEST ADVANCED 8.0 SOFTWARE.

  • LIQUID CHROMATOGRAPHY SYSTEM WITH ÄKTA AVANT 25 FRACTION COLLECTOR.

  • EPPENDORF EPMOTION 5075VT ROBOTIC PIPETTING PLATFORM.

  • AUTOCLAVE TUTTNAUER 3870 MLV.

  • FLUOSTAR OMEGA PLATE READER.

  • CLASS II MICROBIOLOGICAL SAFETY CABINET BIO II ADVANCE PLUS (GREEN LINE).

SERVICES OFFERED
RULES OF USE
RATES (2023)
REQUEST FOR SERVICES

STAFF

CONTACT: lbm (+@irnasa.csic.es)